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Differential cellular gene expression in duck trachea infected with a highly or low pathogenic H5N1 avian influenza virus

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Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells — Experts@Minnesota

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Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells — Penn State

Clin.

  • , Carlson, G.

  • doi:.

  • We here describe the application of suppression subtractive hybridization SSH coupled with cDNA microarray analysis for isolation and identification of chicken transcripts that change in expression on infection of host cells with a paramyxovirus.

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However, it remains tedious and inefficient sequencing works for identifying genes including the great number of redundancy in the subtracted amplicons, and sacrifices the original advantages of high sensitivity of SSH in profiling low-expression transcriptomes.

  • Scand.

  • RNA labeling and microarray procedures In this study, we used the GMRCL Human 15 K set, Version 2 chips as previously described [], which contained 14,811 sequence-verified, human cDNA clones mapped to 12,530 distinct genes.

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